• Requirements: Beaker, visking tube, thistle funnel, sugar solution, water.
• Procedure:

A beaker is filled with water. A visking tube, cellophane or pig’s bladder is tied at the end of a thistle funnel. A 30% sugar solution is then poured into the thistle funnel to come half way up the tube. The thistle funnel is then lowered into a beaker of water and clamped to stand with the level of the sugar solution marked with ink. The set up is left for six hours.

• Results: After six hours, it is seen that the level of sugar in the thistle of funnel has increased while the level of water in the beaker has decreased.
• Explanation: The water in the beaker is hypotonic while the sugar solution in the thistle funnel is hypotonic. Water then moves from the beaker of water to the thistle funnel through the selectively permeable visking tube.
• Conclusion: Osmosis takes place in non-living tissue.